Known as Experiment 626, he is an alien from outer space, one day ends up falling into Earth and lands to the island called Izayoi.
Known as Experiment 624, she is a pink female experiment who is Stitch's love interest. Stitch is madly in love with her and she shares the same feeling.
She is a young 9-year old 4th grader, elementary-school student who lives on a fictional island off the shore of Okinawa, called Izayoi. Living on Izayoi, she learned and studies karate, living a rather normal life, but having to deal with minor problems living with her grandmother, Obaa. However, one day she meets a strange creature that has come from the depths of space, Stitch.
He is an evil genius scientist, the one who created Stitch, and the other 625 Experients. He is assigned by the Galactic Council to watch over Stitch, and lives with Yuna and Obaa. He is much friendlier in this series than the original, and has less of a penchant for evil.
He is a one-eyed alien working for the Galactic Council and Earth expert. He is Jumba's best friend, and lives with him along with Yuna, Obaa, and Stitch.
He/she is a tiny alien who resembles as Pleakley's pet.
Zak and Wheezie They are a two headed dragon who love to play music on their scales and they also have a spaceship that is half green and half purple with their tail on the end of the spaceship.
He is a dimunitive albino alien scientist with a rodent-like appearance with white fur, red eyes, a hamster-like body, a gerbil-like lower body and rabbit-like ears, two feet tall, wearing a red cape with a yellow H on the front, who desires to conquer the galaxy.
Also known as Experiment 625. He is as strong as Stitch, but very lazy, and likes to eat sandwiches.
A new villain, introduced in season 3. She wants Stitch for his Power Chip and forces Dr. Hamsterviel to help her.